Hydrogen peroxide catalase coursework

Microbiology - 008 - Catalase Test

Also, I noticed if the barrel was wet, the syringe often got stuck for a short time before it recorded the volumes of gas. Implementation and Results - It is here that you initially discuss any observations you made when the experiment was going on. The oxygen produced in 30 seconds is collected over water.

Reassemble the apparatus, refill the measuring cylinder, and repeat from g to j with another concentration of hydrogen peroxide. Each piece of apparatus has an apparatus error with an upper and lower limit.

Clean and Dry Equipment I also had to make sure I washed out the conical flask and beaker thoroughly with distilled water and dried them sufficiently. It may not be possible to i Source s: Take care inserting the bung in the conical flask — it needs to be a tight fit, so push and twist the bung in with care.

However, I do not believe the substrate concentrations were significantly different because my repeats were mostly concordant, so a similar amount of oxygen was produced which must mean that there was a similar number of substrate molecules in each concentration.

A stopwatch or any similar device can be used to monitor the time of reaction. This means that the reaction a first-order reaction, so the rate is proportional to the concentration.

Investigating an enzyme-controlled reaction: catalase and hydrogen peroxide concentration

Considering the shape of curve is significant for analysis. Below is a summary of all the percentage errors. To activate the noxious spray, the beetle mixes the contents of the two compartments, causing oxygen to be liberated from hydrogen peroxide.

However, I also believed that if I halved the concentration then the rate of reaction volume of oxygen produced would also be halved, and so the rate would be proportional to the concentration. It is important to remember that working in the classroom may not always allow you to work to this level of accuracy but if you understand and show why your experiments didn't necessarily yield a nice smooth graph then you will get better marks anyway.

I believed this was accurate, but upon reflection, using a pipette would have been much more accurate as pipettes have a much lower apparatus error than volumetric flasks. Michaelis-Menton mechanism of enzyme catalysis. In this procedure, 2 cm3 of 10 vol hydrogen peroxide will release 20 cm3 of oxygen if the reaction goes to completion.

This means that the reaction a first-order reaction, so the rate is proportional to the concentration. Capillary tubes may also be used. I will work out the gradient of these new results and plot them on a new rate of reaction graph.

An intensive metabolic activity can be found in soaked pea seeds as they begin to germinate. This released oxygen can be measured in order to study the enzyme activity.

Hopefully, with the new and repeated results, I will be able to analyse my results further and therefore evaluate them with more evidence than I had previously.

Read up on catalase as a catalyst in this reaction and what factors affect its efficacy. Then the rate of reaction is calculated. This should include some background about the reaction along with some hypothesis as to how you think different things like pH, temperature and concentration will affect the decomposition reaction.

The presence of catalase in bacterial cells depends on both the growth condition and the medium used to grow the cells. Lesson organisation You could run this investigation as a demonstration at two different concentrations, or with groups of students each working with a different concentration of hydrogen peroxide.

Role of Catalase in the Red Blood Cells, in prevention of toxicity from hydrogen peroxide. In order to avoid this toxicity, the enzyme Catalase is present in sufficient quantities in the cells. Streptococcus [47] and Enterococcus spp.

Apparatus and Chemicals For each group of students:. The hydrogen peroxide once in the bloodstream comes in contact with catalase and cytochrome-C which are two enzymes.

Catalase turns the hydrogen peroxide into water and oxygen immediately. The cytochrome-C enzyme, which binds to hydrogen peroxide, is not permitted to become water and oxygen for about forty minutes from first contact. Catalase is an enzyme present in the cells of plants, animals and aerobic (oxygen requiring) bacteria.

It promotes the conversion of hydrogen peroxide, a. AP Biology Lab #2 Enzyme Catalysis Overview: In this lab you will observe the conversion of hydrogen peroxide (H2O2) to water and oxygen gas by the enzyme catalase, and then measure the amount of oxygen generated.

You will also calculate the rate of an enzyme-catalyzed reaction. Apr 30,  · I had to change the volume of hydrogen peroxide used from 5cm 3 to 4cm 3 because the first reaction with % hydrogen peroxide went too fast to collect oxygen at a measurable rate. When I repeated the procedure with 4cm 3 of hydrogen peroxide, I could effectively measure the volume of elleandrblog.coms: 5.

Feb 28,  · Hello, I'm doing my GCSE additional science coursework. And I I'm investigating the rate of reaction of the enzyme Catalase and the substrate: Hydrogen Peroxide.

The independent variable I chose to investigate was the Concentration of enzyme i.e.

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changing the concentration of the elleandrblog.com: Resolved. If the bacteria possess catalase (i.e., are catalase-positive), when a small amount of bacterial isolate is added to hydrogen peroxide, bubbles of oxygen are observed.

Effect of Substrate Concentration on the Rate of Activity of Catalase

The catalase test is done by placing a drop of hydrogen peroxide on a microscope slide.

Hydrogen peroxide catalase coursework
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Hydrogen Peroxide